Can I use a pci-e x16 slot for multple cards?

Yes, a x1 card should work in a x16 slot It's good that you've got it to work in a x1 slot What else have you tried it in besides the x16 slot

You're going to want to use the PCIe x16 slot, this is usually the top one; but it should be labeled in your motherboard manual The other slots

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